Microscope: The LSM 510 confocal system uses a Zeiss upright microscope (AXIO Imager M1) with transmitted (HAL), UV (HBO) and laser illumination sources. It can collect bright field, DIC (using laser as light source) and confocal fluorescence images. The microscope has 10x, 20x (dry), 40x (W), 40x (oil) and 100x (oil) objectives.
Fluorescence Filters: DAPI (blue), Rhodamine (red) and FITC (green).
Laser: Diode laser (405 nm), Argon (458, 477, 488, 514 nm) and HeNe (543, 594, 633 nm).
Software: The LSM5 software, version 3.2, controls the microscope, scanning, laser module, tools and image acquisition and processing.
Computer: A high end PC (Fujitsu Siemens Computers, Celsius) with MS Windows XP operating system.
Capabilities: Z-series, 3-D/rotatable reconstruction, time series for following cellular events, colocalization of multiple proteins and multicolor imaging of fixed and live samples.
Use: Wide array of imaging applications including protein localization analysis, cytoskeletal studies, live cell imaging, calcium signaling and cell cycling studies.