Microscope: This Applied Precision DeltaVision I microscope system uses a standard Olympus IX-71 inverted microscope with a xenon arc lamp as the illumination source for exciting fluorophores from the UV to far Red. It can also collect transmitted light images (bright field and DIC). It has 20x, 40x (dry), 60x and 100x (oil) objectives.
Filter Sets: It contains the filter sets for imaging DAPI, Rhodomine, FITC, GFP, YFP and CY5 fluorophores.
Camera: Image capture is done with CoolSnap HQ2 high resolution CCD camera. It can be used for slow scan and high-resolution work of fixed samples and fast, high sensitivity image capture of live cell samples.
Software: The DeltaVision uses the acquisition software SoftWorx, which can automate the deconvolution computations and can generate 3D reconstructions.
Computer: Uses PC running Redhat Linux (Centos). Windows operate Instrument Controller.
Capabilities: Z-series, 3D/rotatable reconstruction, time course series and multiple-color fluorescence microscopy of fixed and live samples.
Use: Wide array of imaging applications including cytoskeletal studies, live cell imaging, cell cycling studies, protein translocation and protein pathway analysis.
- For further detail consult DeltaVision user manual on the self in room# 163.
- Temperature, humidity and CO2 control can be set up for live cell imaging.