Microscope: This Zeiss LSM 710 confocal system uses a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted (HAL), UV (HBO) and laser illumination sources. It can collect transmitted light images (bright field and DIC) as well as conventional and confocal fluorescence images. The scope has 10x, 20x, 40x (dry), 40x (water), 63x and 100x (oil) objectives.
Fluorescence Filters: DAPI, Rhodomine (red) and FITC (green).
Lasers: 405 nm Diode laser, Argon/2 (458, 488, 514 nm), HeNe1 (543/561 nm) and HeNe2 (633 nm).
Detectors: 34 channel confocal and transmitted light detectors.
Software: ZEN 2011 software to control the microscope, scanning, laser module, tools and the image acquisition and processing.
Computer: LSM710 is controlled via a HP Z800 workstation with the Windows 7 (64 bit) operating system.
Capabilities: Z-series, 3D/rotatable reconstruction, time-lapse analysis for following cellular events in live samples, and co-localization of multiple proteins and multicolor imaging of fixed samples.
Use: Wide array of imaging applications including protein localization analysis, cytoskeletal studies, live cell imaging and cell cycling studies.