Zeiss LSM 980 Confocal Microscope with Airyscan2 and Multiplex Mode

Microscope: The Zeiss LSM 980 Confocal system uses an inverted Axio Observer 7 microscope stand with transmitted (HAL), UV (HBO) and laser (405 – 730 nm) illumination sources. It can collect bright field, DIC (using laser as light source) and confocal fluorescence images.

Objectives: 10x/0.45 WD=2 mm, 20x/0.8 WD=0.55 mm (dry), 40x/1.3 (oil), 63x/1.4 (oil) and 100x /1.4 WD=0.17 mm (oil).

Microscope Fluorescence Filters for Viewing: DAPI (blue), Rhodamine (red) and FITC (green).

Lasers: Diode lasers: (405 nm), (458, 488, 514 nm), (639 nm) and (730 nm). DPSS laser (561 nm).

Software: Zen 3.3 (Blue) software controls the microscope as well as image acquisition and processing.

Computer: HP Z6 G4 R2 with MS Windows 10 operating system.

Capabilities: Z-series, 3-D/rotatable reconstruction, time series for following cellular events, localization of multiple proteins and multicolor imaging of fixed and live samples.

Uses: Wide array of confocal and super resolution imaging applications including protein co-localization, cytoskeletal studies, Tiling of large tissue samples or lot of cells, Z-stack 3D/4D imaging, live cell imaging, calcium signaling and cell cycling studies.